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Item #:
19422815
EMS 21800 (TC-NBT) Thiocarbamyl Nitro-Blue Tetrazolium, 100 mg
Item #:
19422815
AUD 145
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What Stands Out
High Purity
EMS 21800 offers exceptional purity in its thiocarbamyl nitro-blue tetrazolium formulation, ensuring reliable results for laboratory applications and reducing variability in assays.
Versatile Applications
This compound is suitable for various biochemical assays, making it a go-to choice for researchers in fields such as microbiology, biochemistry, and clinical diagnostics.
Convenient Packaging
Packaged in 100 mg amounts, EMS 21800 provides an ideal quantity for small-scale experiments, minimizing waste while maintaining a cost-effective solution for laboratories.
Product Details
- Product Weight: 100 mg
- Sold individually
- Used for Staining & Dye purposes
- Chemical Formula: C42H32Cl2N12O6S2
- Molecular Weight: 935.8
- CAS Number: 36889-43-7
| Item Weight | 0.22 lbs (100 grams) |
Who Should Buy?
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Research Laboratories
Ideal for labs conducting biochemical assays requiring a reliable redox indicator for enzyme activities.
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Academic Institutions
Beneficial for universities and colleges focusing on biochemistry and molecular biology research projects.
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Pharmaceutical Development
Useful in pharmaceutical companies for drug testing and development involving mitochondrial and cellular respiration studies.
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General Consumers
Not intended for everyday consumers as it requires specialized knowledge for proper application and handling.
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Beginner Researchers
Not suitable for novice researchers without prior experience in using biochemical indicators and conducting complex assays.
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Agricultural Use
Not designed for agricultural applications, such as pest control or soil testing in farming environments.
Product Description
EMS 21800 (TC-NBT) Thiocarbamyl Nitro-Blue Tetrazolium, 100 mg
Have any Query? Chat with us
Customer Questions & Answers
-
Question:
What is EMS 21800 TC-NBT Thiocarbamyl Nitro-Blue Tetrazolium used for?
Answer: EMS 21800 TC-NBT is primarily used as a biochemical reagent in laboratory settings, particularly in assays relating to cell viability and reductase activity. This compound acts as a substrate for various enzymatic reactions, making it essential in the study of cellular processes. For example, researchers utilize it in experiments to measure the metabolic activity of cells, which is crucial for drug testing or assessing the effects of various compounds on cell health. -
Question:
How is EMS 21800 TC-NBT typically prepared for experiments?
Answer: To prepare EMS 21800 TC-NBT for experiments, you need to dissolve the reagent in a suitable solvent, usually dimethyl sulfoxide (DMSO) or phosphate-buffered saline (PBS), to achieve the desired concentration. This solubilization allows for consistent and accurate experimental results. Many laboratories prefer preparing fresh solutions to maintain the reagent's efficacy and stability, particularly in kinetic assays where precise timing is crucial. -
Question:
What safety precautions should be taken when handling EMS 21800 TC-NBT?
Answer: When handling EMS 21800 TC-NBT, it's essential to wear appropriate personal protective equipment (PPE), including gloves, lab coats, and safety goggles. This reagent can pose potential hazards if ingested or inhaled, so working in a fume hood or well-ventilated area is recommended. Familiarity with material safety data sheets (MSDS) is also advisable to understand the compound’s health effects and necessary emergency procedures. -
Question:
Can EMS 21800 TC-NBT be used in combination with other reagents?
Answer: Yes, EMS 21800 TC-NBT can often be used in conjunction with other reagents to diversify its applications in research. For instance, it can be combined with various biological markers to amplify detection in assays. Researchers frequently utilize this technique to enhance sensitivity when examining cellular responses to treatments, enabling more detailed analyses in studies of cellular metabolism and drug effects. -
Question:
What is the significance of the 100 mg packaging of EMS 21800 TC-NBT?
Answer: The 100 mg packaging of EMS 21800 TC-NBT is ideal for laboratory use due to its manageable quantity for various experiments. This amount allows researchers to conduct multiple assays without the concern of the reagent degrading over time or running out prematurely. With this packaging, it's possible for labs to efficiently plan their experimental designs, ensuring they have adequate supplies to obtain reliable data in their studies. -
Question:
Is EMS 21800 TC-NBT suitable for both in vitro and in vivo studies?
Answer: EMS 21800 TC-NBT is primarily utilized in in vitro studies, where it assists in assessing cellular activities and responses in cultured cells. While its application in in vivo studies is limited, researchers may use it in specific situations as a diagnostic tool to measure certain biological activities within living organisms. It's crucial to validate its use in vivo through extensive preliminary studies to ensure accuracy and safety. -
Question:
What methods can be employed to detect the results using EMS 21800 TC-NBT?
Answer: The results of assays using EMS 21800 TC-NBT can typically be detected through spectrophotometric analysis. By measuring absorbance at specific wavelengths post-reaction, researchers can quantify cellular activity and viability. For example, this method is widely used in cytotoxicity assays to evaluate the effectiveness of therapeutic compounds. The precise absorbance values correlate to cell viability, providing clear insights into the experimental outcomes. -
Question:
Are there any alternatives to EMS 21800 TC-NBT?
Answer: Yes, there are several alternatives to EMS 21800 TC-NBT for similar biochemical analyses, such as MTT or XTT assays. Each alternative has unique benefits and may be suitable depending on the specifics of your assay requirements. For instance, MTT is often favored for its simplicity and quick processing, while other compounds might provide different sensitivity levels or spectra, allowing researchers flexibility when choosing the best tool for their experiments. -
Question:
How long can EMS 21800 TC-NBT be stored without losing effectiveness?
Answer: EMS 21800 TC-NBT should be stored in a cool, dry place and typically remains effective for several months, depending on storage conditions. Proper storage is crucial as exposure to light and moisture can degrade the compound. For optimal longevity, it’s advisable to keep the reagent in its original packaging and to seal it tightly after each use. Researchers should always check for changes in color or consistency before use as an additional measure of its viability. -
Question:
Where can I buy EMS 21800 TC-NBT Thiocarbamyl Nitro-Blue Tetrazolium, 100 mg?
Answer: You can buy EMS 21800 TC-NBT Thiocarbamyl Nitro-Blue Tetrazolium, 100 mg from Ubuy in Australia. Ubuy is a trusted online retailer that stocks a wide range of laboratory reagents and supplies, ensuring that you can easily find the products you need for your research. They offer a straightforward online shopping experience and often have stock readily available for quick procurement.
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